Tool: Zeiss confocal laser scanning fluorescent microscope LSM 800 MAT
Organization
Institute for the Protection of Cultural Heritage of Slovenia
Last checked date
05-12-2024
Description
ZEISS LSM 800, the confocal laser scanning microscope (CLSM) can image fluorescence in thin optical slices and stacks of images can be acquired to examine fluorescent structures in 3D. The system is based on a Axio Imager Z2m microscope and is equipped with a URGB laser module (Diode lasers: 405, 488, 561 and 640 nm), high-quality objectives (10x, 20x, 40x water, 40x oil, 63x oil) and three sensitive GaAsP detectors. Can characterize precision topography of 3D microstructures surfaces by combining all essential light microscopy contrasting techniques. When the HXP120V reflective light source is active the system will accure images in the widefield foulrescence mode, hwoever when the LM laser unit is acitve the system will generate 3D models of the scanned sample.
Output and data types
Image
Image/False color
Dataset
Model/3D
Impact on object or sample
Micro-destructive (removal or permanent alteration of very small, inconspicuous portions)
Microinvasive (very minor, controlled, and reversible physical interaction)
Nondestructive (no permanent damage or alteration)
Noninvasive (no physical alteration)
Manufacturer
Carl Zeiss Microscopy GmbH, Carl-Zeiss-Promenade 1007745 Jena, Germany
Acquisition areas
large spot
Working distances
Near-contact (< 0.01 m)