Tool: Raman microspectrometer
Organization
Hungarian National Museum Public Collection Centre, Hungarian National Museum
Last checked date
13-12-2024
Description
Raman spectroscopy is a non-invasive and non-destructive analytical technique, which provides insights into the molecular composition and vibrational modes of matter. This method involves illuminating a sample with monochromatic visible light (with lasers). When light interacts with the molecules in an object, some of the light scatters inelastically, a phenomenon called Raman scattering. Unlike traditional spectroscopy, Raman scattering observes changes in energy levels of scattered photons. These energy shifts are related to the vibrational and rotational motions of molecules within the sample, offering a molecular fingerprint of the substance. These fingerprints need to be compared to a database, to determine the composition and structure of various substances.
In Our laboratory a Renishaw InVia Qontor Raman microspectrometer is in operation since 2023.
Specifications:
Lasers supported 532, 633 and 785 nm
Laser gratings of 1200 and 2400 grooves/mm
Spectral resolution 0.3 cm-1 (FWHM)
Stability < ±0.01 cm-1
Leica microscope with 10x, 20x 50x and 100x objectives
Spatial resolution (lateral) 0.25 µm
Spatial resolution (axial) < 1 µm
Qontor system - with full automation and focus tracking technology
High resolution and fast (up to 1000 spectra/s) point and area mapping
Flexible sampling arm for bigger samples which cannot fit the microscope stage
Option for polarized Raman spectroscopy
Output and data types
Image
Dataset/2D
Dataset/3D
Model/3D
spectra in *.csv
data interpretation
Impact on object or sample
Noncontact (no physical contact)
Nondestructive (no permanent damage or alteration)
Noninvasive (no physical alteration)
Acquisition areas
small spot
micro
2 x 5 cm
Working distances
Near-contact (< 0.01 m)
Close-range (0.01 - 0.1 m)